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Further immunoblotting analysis of the nitration level of HGF was performed in the same manner using anti-nitroY198/Y250-HGF mAbs (1:2500 dilution,clones 3A11C6 and 6B82C3 raised in-house specifically to visualize nitroY198-HGF and nitroY250-HGF, respectively, see Elgaabari et al

With age as a covariate, the Spearman rho values remained practically unchanged (differed by less than 0.02) regardless of if we excluded or included the covariate, separately for all 4 conditions [Hypothyroidism/CFS x Before/After L-carnitine]

The goal isn't to zero out oxidative stress

However, for less reactive ROS the results obtained correlate better with other methods