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The colony regions were defined by applying an automated thresholding algorithm (Otsu method)

Potential Risks and Side Effects While glutathione treatments are generally considered safe, some individuals may experience side effects, including: Allergic reactions (rare) Low blood pressure (with IV administration) Gastrointestinal issues, such as nausea or diarrhea (more common with oral supplements) It is essential to consult with a healthcare professional before starting any glutathione treatment, especially for individuals with underlying health conditions or those who are pregnant or breastfeeding

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