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the pool containing Arg 34 GLP-1 (7-37) and the truncated form, Arg 34 GLP-1 (9-37) , as an impurity was adjusted to pH 3.1, and 5 ml of the resulting solution was applied to a 20 ml Source 30S (Amersham Pharmacia Biotech) column equilibrated with 100 ml 0.85 % (w/w) tri-sodium citrate, 0.33 % (w/w) succinic acid, 0.6 % (w/w) di-sodium hydrogen phosphate di-hydrate, 45 % (w/w) ethanol, pH 3.2

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10.1038/s41598-018-26296-6 86 RibasV.Garca-RuizC.Fernndez-ChecaJ

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