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oxidation of iodide, iodination of tyrosine residues of thyroglobulin (Tg), and the oxidative coupling of iodinated tyrosine residues to generate Tg-bound TH, are catalyzed by the enzyme thyroid peroxidase (TPO), which is located in the apical membrane of the thyrocyte generating hydrogen peroxide (H2O2) as a substrate [61, 62]
The compiled evidence was discussed during the second virtual meeting, and additional references were incorporated based on panel recommendations
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The precipitate was collected (6000 rpm, 30 min, 4 C) and washed with 1 mL of acetone three times to remove the TCA