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After incubation with the primary antibody, the cells were washed with PBST and stained with secondary antibodies (Goat Anti-Rabbit IgG H&L Alexa Fluor 488, Abcam, ab150077
Overall, Nrf2 is fundamental for the sustenance of the GSH redox state through glutathione reductase transcriptional regulation and for cell protection against oxidative stress (Harvey et al., 2009)
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The fear that this might be permanent
Its considered experimental, and its safety and efficacy profiles have not been firmly established through large-scale clinical trials
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