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Samples were vortexed and left at 20 C for 1 h, centrifuged for 10 min at 21,130 g and 4 C and transferred to glass vials for their analysis by LCMS

Plain sterile water is not preservative-free bacteriostatic once the vial seal is broken, it is susceptible to microbial contamination and should only be used for single-draw applications

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