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The cells were sub-cultured, when the cells are 80% confluent, using a cell scraper (AGC Techno Glass Co., Ltd) after processing by DPBS() (no calcium, no magnesium, Thermo Fisher Scientific Inc.) and CTK solution

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The peptide has been studied extensively in animal models since the 1990s, with over 100 published research papers examining its effects on various tissues and conditions

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2,7-dichlorofluorescin-diacetate (DCFDA) was used for reactive oxygen species (ROS) measurement